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Home / Encyclopedia / Handling & Reference / Bacteriostatic water &
MONOGRAPH No. 041
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Handling & Reference

Bacteriostatic water & sterile technique

Bacteriostatic water is not the same as sterile water, saline, or water for injection. Using the wrong reconstitution liquid is one of the most common handling mistakes. This guide explains what BAC water is and why it matters.

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What is bacteriostatic water?

Bacteriostatic water is sterile water for injection containing 0.9% benzyl alcohol as a preservative. Benzyl alcohol inhibits bacterial growth — it does not kill bacteria already present, but prevents new bacteria from colonising the solution after the vial is opened. This makes it suitable for multi-dose vials: unlike plain sterile water, BAC water remains stable for research use after being punctured multiple times.

RECONSTITUTION LIQUIDS COMPARED
LIQUIDPRESERVATIVEMULTI-DOSE?NOTES
BAC water0.9% benzyl alcoholYesStandard for peptide reconstitution
Sterile WFINoneSingle-useUse entire vial at once
0.9% salineNoneSingle-useAcceptable alternative same session
Tap / distilledNoneNeverNot sterile — do not use

5-step sterile technique

1
Clean workspace with 70% IPA — let dry
Wet IPA does not disinfect. Allow 30 seconds to air-dry.
2
Swab both vial tops — let dry
One fresh alcohol swab per vial. Both BAC water and peptide vial.
3
Never touch the needle tip
Handle syringes by the barrel only. Contaminated needle = contaminated vial.
4
Add BAC water slowly down the glass wall
Never inject directly onto the cake. Swirl gently — do not shake.
5
Store reconstituted vials at 2–8°C immediately
Refrigerate, do not freeze. Use within 4–6 weeks (vendor BUD).
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Potency retained over time

Each line is one storage condition: it starts at 100% and falls as potency is lost. Above the dashed 80% line the material is considered reliable — hover for exact values.

Freezer −20 °C
n/a
Unopened · long shelf life
Fridge 4 °C
open: wks
Opened · use within weeks
Room 20 °C
open: wks
Stable · keep sterile
Warm 37 °C+
store cool
Keep cool · away from heat

⚠ Modelled per compound from molecular structure — chain length, cyclisation, oxidation- and deamidation-prone residues and light sensitivity. Indicative only, not lab-measured per batch.

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