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MONOGRAPH No. 111
EVIDENCE BASEModerate
SEQUENCE
9 amino acids (zinc-dependent)
MW
858.9 g/mol (peptide, excl. Zn)
CAS
63958-90-7
EU STATUS
Research only
WADA
Not listed
MIN PURITY
≥98% HPLC
⏳ Anti-Aging & Longevity

Thymulin — complete EU guide.

Thymulin (formerly FTS, facteur thymique sérique) is a zinc-dependent nonapeptide hormone produced by the thymus, discovered in 1977 and among the most thoroughly characterised thymic peptides in the immunology literature.

Last reviewed:
Key findings at a glance
  • Zinc-dependent nonapeptide (pGlu-Ala-Lys-Ser-Gln-Gly-Gly-Ser-Asn) produced exclusively by thymic epithelial cells; biologically inactive without bound zinc.
  • Discovered in 1977 by Bach and Dardenne at the Institut Necker, Paris — a distinct research lineage from the Russian Khavinson bioregulators (e.g. Thymalin, Vilon) covered elsewhere in this encyclopedia.
  • Best-characterised role: induction of T-cell differentiation markers and modulation of T-cell subset function in animal and in vitro models.
  • A synthetic peptide analogue (PAT) has been studied separately for neuroprotective and analgesic effects in animal inflammation models.
  • No completed modern human clinical trials; most mechanistic work dates from the 1980s–1990s French immunology literature.

What is Thymulin?

Thymulin, originally named facteur thymique sérique (FTS, 'serum thymic factor'), is a nonapeptide hormone produced exclusively by thymic epithelial cells. It was discovered in 1977 by Jean-François Bach and Mireille Dardenne at the Institut Necker in Paris. Its defining feature is a strict requirement for zinc: the zinc-free peptide (FTS) is biologically inactive, while the zinc-bound form (thymulin, or Zn-FTS) is the active hormone. This is distinct from the Khavinson-school Russian bioregulators (Thymalin, Vilon, Thymagen) covered elsewhere in this encyclopedia, which come from a separate research tradition.

How is it proposed to work?

Once bound to zinc, thymulin adopts a specific three-dimensional conformation, confirmed by NMR, that allows it to bind receptors on lymphocytes and induce T-cell differentiation markers. Research has focused on its role in T-cell maturation, modulation of natural killer cell activity, and a feedback loop with the hypothalamic-pituitary axis — circulating thymulin follows a circadian rhythm linked to ACTH levels.

What does the research show?

Thymulin is one of the best-characterised thymic hormones in the immunology literature, with a substantial body of animal and in vitro work from the 1980s and 1990s establishing its zinc-dependent structure and T-cell effects. A separate line of research has studied a peptide analogue of thymulin (PAT) for neuroprotective and analgesic properties in animal models of inflammation. However, most of this evidence predates modern clinical-trial standards, and no completed contemporary human trials exist.

EU legal status

Thymulin is not an approved medicine in the EU or elsewhere. It is sold by research-chemical suppliers as a laboratory peptide, research use only, and is not listed on WADA's prohibited list.

EVIDENCE SUMMARY
MODERATE
T-cell differentiation induction — Foundational 1977 characterisation and follow-up work established that zinc-bound thymulin induces T-cell surface markers in animal and in vitro models.
MODERATE
Neuroprotective/analgesic analogue — A synthetic peptide analogue of thymulin (PAT) reduced markers of neuroinflammation and showed analgesic effects in animal models, a separate line of work from its immune actions.
LIMITED
Human clinical evidence — Most human data comes from small, older observational studies (e.g. lower thymulin in anorexia nervosa patients); no completed modern controlled human trials exist.
Most thymulin research predates modern clinical-trial standards and comes from animal or in vitro models. Its zinc-dependent activity means research handling requires attention to buffer composition. Not an approved medicine; no human dosing data exists.
✓ PeptideCompare provides factual, non-promotional context on Thymulin's mechanism, discovery history and evidence base — not dosing or administration advice.

Molecular information

Molecular formula
C33H54N12O15
Molecular weight
858.9 g/mol (peptide, excl. Zn)
CAS number
63958-90-7

Pharmacokinetics

No established human pharmacokinetic data. Published human PK parameters for this compound are not available; reported data are limited to animal models or absent. No curve is shown, to avoid implying data that does not exist.

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Frequently asked questions

What is Thymulin?

Thymulin (formerly FTS) is a zinc-dependent nonapeptide hormone produced by thymic epithelial cells, discovered in 1977. It requires bound zinc to be biologically active.

Is Thymulin the same as Thymalin or Thymagen?

No. Thymulin comes from a separate French research lineage (Bach and Dardenne, Institut Necker), distinct from the Russian Khavinson bioregulator family that includes Thymalin, Vilon and Thymagen.

What is Thymulin studied for?

Its best-characterised role is inducing T-cell differentiation markers and modulating T-cell subset function in animal and in vitro immunology research. A separate peptide analogue (PAT) has been studied for neuroprotective effects.

Why does Thymulin need zinc?

The zinc-free peptide (FTS) is biologically inactive. Zinc binding induces the specific three-dimensional conformation required for receptor activity — a defining and unusual feature among thymic peptides.

Is Thymulin approved as a medicine?

No. It has no approved medical indication in the EU or elsewhere and is sold strictly as a research chemical.

Is Thymulin banned in sport?

No. It does not appear on WADA's prohibited list.

How do I verify Thymulin quality?

Require a batch-specific COA from a named third-party lab confirming identity by mass spectrometry and purity by HPLC (≥98%).

References

  1. Original biochemical characterisation of the serum thymic factor (later named thymulin) by Bach and colleagues at the Institut Necker. Bach JF, et al. Nature. 1977;266(5597):55–57. DOI PubMed 300146
  2. Review establishing the equimolar zinc requirement for thymulin's biological activity, confirmed by NMR structural studies. Dardenne M, Pléau JM. Res Immunol. 1994;145(3):233–235. DOI PubMed 18476235
  3. Study of a thymulin-related peptide analogue (PAT) showing analgesic and anti-inflammatory effects in a neuropathic pain model. Safieh-Garabedian B, et al. Neurosci Lett. 2019;702:61–65. DOI PubMed 30503917

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