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MONOGRAPH No. 068
TYPE
Nucleotide analogue / not a peptide
MW
258.23 g/mol
CAS
2627-69-2
EU STATUS
Research only
WADA
Prohibited S4 (AMPK activators)
MIN PURITY
≥98% HPLC
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AICAR — EU research guide.

AICAR (5-aminoimidazole-4-carboxamide ribonucleotide) is a cell-permeable AMP-mimetic that directly activates AMPK, the master metabolic switch. It is widely used in metabolic research and is prohibited by WADA.

Last reviewed:

What is AICAR?

AICAR is converted intracellularly to ZMP, an AMP mimetic that activates AMPK by simulating energy depletion. AMPK activation switches cells from anabolic to catabolic metabolism: increasing fatty acid oxidation, glucose uptake, mitochondrial biogenesis and reducing mTOR signalling. Note: AICAR is not a peptide but a nucleotide derivative; it appears in peptide research databases due to its common co-use in metabolic research.

What does the research show?

Seminal mouse studies (Evans lab, Salk Institute) showed ~44% improvement in running endurance with AICAR alone, without exercise. Extensive use in diabetes, obesity and mitochondrial disease research. AICAR activates PGC-1α and increases GLUT4 expression. The exercise-mimicking profile attracted significant sports doping research interest. No approved human therapeutic as of mid-2026.

EU legal status

Not approved as a medicine in the EU. WADA-prohibited in sport (S4 — Hormone and metabolic modulators). Available for laboratory metabolic research only.

WADA-prohibited. Not a peptide — nucleotide derivative. No human therapeutic approval. Not for human use or use in competitive athletes. This is a research compound, not a medication.
✓ PeptideCompare lists AICAR from EU research vendors. WADA prohibition status should be clearly noted on any research documentation.

Molecular information

Molecular formula
C9H14N4O5
Molecular weight
258.23 g/mol
CAS number
2627-69-2
Source: PubChem CID 17513

Pharmacokinetics

No established human pharmacokinetic data. Published human PK parameters for this compound are not available; reported data are limited to animal models or absent. No curve is shown, to avoid implying data that does not exist.

AICAR across EU suppliers

COA-verified EU vendors · Updated monthly

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